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RNA Detection and Subtype C Assessment of HIV-1 in Infants with Diarrhea in Ethiopia
E-Mail Address of Dr. Workenesh Ayele
g.pollakis@amc.uva.nl
E-Mail Address of Dr. Tsehai Assefa
g.pollakis@amc.uva.nl
E-Mail Address of Dr. Sileshi Lulseged
g.pollakis@amc.uva.nl
E-Mail Address of Dr. Belete Tegbaru
g.pollakis@amc.uva.nl
E-Mail Address of Dr. Hiwot Berhanu
g.pollakis@amc.uva.nl
E-Mail Address of Dr. Wegene Tamene
g.pollakis@amc.uva.nl
E-Mail Address of Dr. Zenit Ahmedin
g.pollakis@amc.uva.nl
E-Mail Address of Dr. Birzaf W Tensai
g.pollakis@amc.uva.nl
E-Mail Address of Dr. Mengistu Tafesse
g.pollakis@amc.uva.nl
E-Mail Address of Dr. Jaap Goudsmit
g.pollakis@amc.uva.nl
E-Mail Address of Dr. Ben Berkhout
g.pollakis@amc.uva.nl
E-Mail Address of Dr. William A Paxton
g.pollakis@amc.uva.nl
E-Mail Address of Dr. Michel P deBaar
g.pollakis@amc.uva.nl
E-Mail Address of Dr. Tsehaynesh Messele
g.pollakis@amc.uva.nl

E-Mail Address of Dr. Georgios Pollakis
g.pollakis@amc.uva.nl
Abstract
In the absence of chemoprophylaxis, HIV-1 transmission occurs in 13-42% of infants born to HIV-1 positive mothers. All exposed infants acquire maternal HIV-1 antibodies that persist for up to 15 months, thereby hampering diagnosis. In resource limited settings, clinical symptoms are the indices of established infection against validated laboratorybased markers. Here we enrolled 1200 children hospitalized for diarrheal and other illnesses. 20-25% of those tested, aged 15 months or younger, were found to be HIV-1-seropositive. Where sufficient plasma was available, HIV-1 RNA detection was performed using a subtype-insensitive assay, with 71.1% of seropositive infants presenting with diarrhea showing positive. From sub-typing analysis, we identified that viruses of the C’ sub-cluster were predominated amongst infants. Although this study may overestimate the HIV-1 frequency through testing symptomatic infants, diarrhea can be seen as a useful marker indicating HIV-1 infection in infants less than 15 months old.
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Ayele W, Assefa T, Lulseged S, Tegbaru B, Berhanu H, Tamene W, Ahmedin Z, Tensai B, Tafesse M, Goudsmit J, Berkhout B, Paxton W, deBaar M, Messele T, Pollakis G. RNA Detection and Subtype C Assessment of HIV-1 in Infants with Diarrhea in Ethiopia . Open AIDS J, 2009; 3: . http://dx.doi.org/10.2174/1874613600903010019Article History
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Ayele W, Assefa T, Lulseged S, Tegbaru B, Berhanu H, Tamene W, Ahmedin Z, Tensai B, Tafesse M, Goudsmit J, Berkhout B, Paxton W, deBaar M, Messele T, Pollakis G. RNA Detection and Subtype C Assessment of HIV-1 in Infants with Diarrhea in Ethiopia . Open AIDS J, 2009; 3: . http://dx.doi.org/10.2174/1874613600903010019Export Citation
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© Ayele .; Licensee .et al
Open-Access License: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Address correspondence to this author at the Laboratory of Experimental Virology (LEV), University of Amsterdam, 1105 AZ Amsterdam, The Netherlands; Tel: 31-20-566-8572; Fax: 31-20-691-6531; E-mail: g.pollakis@amc.uva.nl
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